Volume 1, Issue 2 (2014)
Research Article
BIOCHEMICAL PROFILING OF LENTIL (Lens culinaris Medik) GERMPLASM AT DIFFERENT GROWTH STAGES
Amit Kumar Singh, Pankaj Kumar, Jitender Singh, Rosy Rani, Anchal Rani, Pradeep Shukla and Pragati Misra
Abstract: The lentil (Lens culinaris MediK) is an annual, bushy herb, erect or sub-erect with many branches. The genus name Lens is derived from the typical shape of the lentil seed. It is one of the most nutritious of all pulses that are traditionally grown in Central and Southwest Asia. Lentil is a self-pollinating diploid (2n = 14 chromosomes) legume crop that is highly valued as a high protein food. For this biochemical profiling analysis study seventy six lentil germplasm were collected from different sources to characterize the germplasm based on biomolecules concentration. Biochemical parameters viz. total sugar content estimation and protein content estimations were studied in this study at different
growth intervals.
Research Article
PROBIOTIC ACTIVITIES OF LACTIC ACID BACTERIA ISOLATED FROM HUMAN BREAST MILK
Ashwani Kumar Singh, Abhishek Pandey, Megha Sharma, Komudi, and Anupam Singh
Abstract: Human breast milk contains potentially Probiotic lactic acid bacteria. Studies showed that this fluid has beneficial effects on the health of neonates. It is a complex species-specific biological fluid adapted to satisfy the nutritional requirements of the rapidly growing infants. These bacteria could protect the infant against infections and contribute to the maturation of the immune system. In the present studies Isolates were identified by biochemical tests and their characterization is performed by Probiotic behavior of Lactic acid bacteria.
Research Article
EVALUATION OF DEVELOPED MULTIPLEX PCR TECHNIQUE OF STAPHYLOCOCCUS AUREUS FOR SPECIFICITY AND ITS EFFICIENCY ON FOOD SAMPLES
D. Gayathri and M. Shiva Prakash
Abstract: Staphylococcus aureus is one of the most significant pathogens causing community acquired infections. Among the secreted Staphylococcus virulence factors, there is growing list of enterotoxins which can induce gastroenteric syndrome and toxic shock syndrome. There are up to 15 staphylococcal enterotoxins known but not all play a role in food poisoning. This study focuses on only five enterotoxins that are SEA, SEB, SEC, SED and SEE. Staphylococcal food borne diseases resulting from consumption of food contaminated with Staphylococcal enterotoxins (SEs) produced by certain strains of Staphylococcus aureus. Analytical methods are essential for routine monitoring purposes and safeguard public health. This new technique will reduce the time involved in identification and subsequently evaluation of their toxicity. Therefore early identification by this method will help in minimizing the infections, thereby preventing diarrheal diseases. The evaluation for specificity of mPCR method for Staphylococcus aureus was done by SDS PAGE and confirmed by western blotting with specific antibodies against SEA, SEB, SEC, SED and SEE Staphylococcal enterotoxins. The efficiency of mPCR method was tested on live food samples and identified based on the band length and markers. This technique will be useful in quality control of food products produced by food and dairy industries, food testing laboratories and medical diagnostic laboratories especially at health centers and at field level.
Review Article
MACHINE PERCEPTION IN BIOMEDICAL APPLICATIONS: AN INTRODUCTION AND REVIEW
Munendra Singh, Rohit Kumar Verma, Gaurav Kumar, and Shefali Singh
Abstract: Rapid diagnosis, need of early prediction and control of diseases shifted the biomedical interest towards Artificial Intelligence (AI). This prominently fosters the collaborative interactions of machine learning in biomedical engineering. AI aims at mining relevant information directly from the derivative physiological data, leads to better performance in comparison to human that is suffered by status of mind and mental variability. Evolutions of new machine learning algorithms are helping the even disrupted pattern of raw physiological data in terms of better classification. The purpose of this study is to create the interest of new researchers in this field by understand the basic technique and applications to aid in their research further.
Research Article
DESIGNING OF PORTABLE AND LOW COST ECG SIGNAL ACQUISITION SYSTEM
Uma Shanker Yadav, Utkarsh Pancholi, Aman Tugnawat, Ranjana Patnaik
Abstract: The accurate Electrocardiogram (ECG) and heart rate information is one of the vital aspects for the different kinds of heart functioning disorder identification. The work proposed a universal portable, low cost system to acquire ECGs. A very simple algorithm is developed which can compensate base line shift, high frequency noise present in the ECG, peak detection without losing any information along with removing some artifacts. The algorithm accomplished with six order polynomial fitting curve for the purpose of removing baseline shift and digital filtering techniques are applied to overcome with high frequency noise and artifacts. The value of the real time heart rate is displayed in digital format along with the ECG if the system is connected with computer.